eva‎luation of inhibitory effect of (Marrubium Vulgar l.) extract on ochratoxin A producing pnicillium fungus In licorice root under laboratory conditions.

رعيت محمد حسين Raeiyyat Mohammad hossein

غلام نژااد جلال Gholamnegad Jalal , محمد جمال سحرخيز saharkhiz Mohammad jamal

علوم و مهندسي باغباني گرايش درختان ميوه

كارشناسي ارشد

كشاورزي و منابع طبيعي

علوم و مهندسي باغباني

اكراتوكسين¬آ، شيرين¬بيان، فراسيون¬سفيد، قارچ Penicillium sp.

Ochratoxin A، Glycyhirriza glabra، Marrubium vulgar، Penicillium sp.

چكيده هدف از اين مطالعه بررسي اثر عصاره فراسيون¬سفيد (Marrubium vulgar) روي قارچ Penicillium sp مولد اكراتوكسين¬آ در ريشه شيرين¬بيان در شرايط آزمايشگاهي بود. بدين لحاظ تحقيقي بر روي عصاره فراسيون-سفيد روي بازدارندگي از رشد قارچ¬هاي جدا شده از ريشه شيرين¬بيان (Glycyrrhiza glabra) انجام شد.در اين تحقيق ريشه شيرين¬بيان از انبار سوله¬اي يك شركت توليدي عصاره شيرين¬بيان معتبر در شيراز نمونه¬برداري شد. آناليزهاي مرتبط با ريشه گياه شيرين¬بيان از جمله ميزان اكراتوكسين¬آ وميزان گليسيريزيك¬اسيد به وسيله دستگاه كراماتوگرافي مايع با كارايي بالا (HPLC) و همچنين ميزان رطوبت، ميزان قند كل و ميزان عصاره طبق استاندارد انجام شد. قارچ¬هاي ريشه شيرين¬بيان جداسازي و خالص سپس شناسايي شدند.گياه فراسيو¬ن¬سفيد از كوه دراك شيراز جمع¬آوري و توسط بخش گياه¬شناسي دانشگاه علوم شيراز شناسايي و نمونه¬اي در هرباريوم اين دانشگاه نگه¬داري شد.100 گرم از اندام هوايي گياه فراسيون¬سفيد پس از آسياب¬شدن به ميزان چهار برابر وزن آن اتانول 80 درصد اضافه شد. رطوبت¬هاي اضافي با دستگاه روتاري گرفته شده و سپس از عصاره خشك غلظت¬هاي 500، 1000، 1500، 2000 پي پي ام تهيه شد. سپس ميزان بازدارندگي عصاره فراسيون¬سفيد روي قارچ¬هاي جداسازي شده از ريشه شيرين¬بيان كه شامل دو گونه Aspergillus sp، (Aspergillus niger) و (Aspergillus flavus) ويك گونه Penicillium sp به روش اختلاط با محيط كشت انجام شد كه عصاره فراسيون¬سفيد با غلظت 2000 پي¬پي¬ام در روز پنجم بيش¬ترين درصد بازدارندگي و كم¬ترين درصد بازدارندگي بعد از شاهد مربوط به غلظت 500 و 1000 پي¬پي¬ام در روز اول براي قارچ Aspergillus niger و بيش¬ترين درصد بازدارندگي مربوط به غلظلت 2000 پي¬پي¬ام در روز پنجم وكم¬ترين درصد بازدارندگي 500 پي¬پي¬ام در روز اول براي قارچ Aspergillus flavus و بيش¬ترين درصد بازدارندگي مربوط به غلظت 1000 پي¬پي¬ام در روز دوم و كم¬ترين درصد بازدارندگي مربوط به غلظت 500 پي¬پي¬ام در روز اول براي قارچ Penicillium sp را داشت. عصاره فراسيون¬سفيد بيش¬ترين در صد بازدارندگي از رشد روي قارچ Penicillium sp را داشت. كلمات كليدي: اكراتوكسين¬آ، شيرين¬بيان، فراسيون¬سفيد، قارچ Penicillium sp.

eva‎luation of inhibitory effect of (Marrubium Vulgar l.) extract on ochratoxin A producing Penicillium fungus In licorice root under in vitro Abstract The aim of this study is to investigate the effect of Marrubium vulgare extract on the fungus Penicillium sp., the producer of ochratoxin-A in licorice root (Glycyrrhiza glabra), in laboratory conditions. Thus, a research was conducted on the extract of Marrubium vulgare on the inhibition of the growth of fungi isolated from licorice root. Based on this, licorice root was sampled from the warehouse of a valid company in the field of licorice extract production in Shiraz. Analyzes related to the root of licorice plant, including the amount of ochratoxin-A and glycyrrhizic acid, were performed using a high-performance liquid chromatography (HPLC), as well as moisture level, total sugar, and extract rate according to the standard of the Institute of Standards and Industrial Research of Iran was measured. Fungi grown in licorice root were isolated and purified and then identified. Marrubium vulgar plant was collected from Derak mountain located in Shiraz and identified by biology department of Faculty of Science of Shiraz University and a sample was kept in the herbarium of this university. 100 grams of Marrubium vulgare aerial parts were powdered and 400 grams of 80% ethanol was added to it. Excess moisture was taken with a rotary instrument and then 500 ppm, 1000 ppm, 1500ppm and 2000 ppm concentrations were prepared from the dry extract. In the following, the growth inhibition rate of Marrubium vulgar extract on fungi isolated from licorice root including two Aspergillus sp. (Aspergillus niger and Aspergillus flavus) and one Penicillium sp. was measured by mixing with culture medium. In the case of Aspergillus niger, Marrubium vulgar extract with a concentration of 2000 ppm on the fifth day showed the highest percentage of inhibition, and the lowest percentage of inhibition compared to the control was related to the concentration of 500 ppm and 1000 ppm on the first day. The highest percentage of inhibition in the case of Aspergillus flavus fungus was provided by concentration of 2000 ppm on the fifth day and the lowest percentage of inhibition by 500 ppm on the first day. The highest percentage of inhibition was related to the concentration of 1000 ppm on the second day and the lowest percentage of inhibition was related to the concentration of 500 ppm on the first day for Penicillium sp. By comparing the inhibitory effect of Marrubium vulgar extract on the inhibition of the investigated fungal species, it was found that it had the highest percentage of inhibition of the growth of Penicillium sp. Key words: Ochratoxin A، Glycyhirriza glabra، Marrubium vulgar، Penicillium sp.

10/22/2022 12:00:00 AM

619

HS63

محمد حسين رعيت