In recent years, due to the side effects of chemical drugs in the treatment of leishmaniasis, the use of herbal compounds to treat leishmaniasis wounds has been increasingly expanded. Many researchers have investigated the effects of extracts and essential oils of different native and local plants from different regions of the world on Leishmania in vitro and in vivo. Iran is the main habitat of Ferula assa– foetida L. and on the other hand, the therapeutic effects of this plant on various pathogens, including parasites, have been proven; this study aimed to eva‎luate the therapeutic effect of the Ferula assa– foetida L. stem and root essential oil in treatment and healing of cutaneous leishmaniasis wounds in laboratory mice. The essential oil of the stem and root of the Ferula assa– foetida L. was extracted using a Cleonger machine. RPMI1640 medium was used to culture Leishmania major promastigotes. One tenth milliliter of solution containing parasite was injected subcutaneously at the base of the tails of BALB/c mice using an insulin syringe. After the appearance of a wound at the injection site, treatment with concentrations of 2.5, 10, and 20µg of the stem and root essence of Ferula assa– foetida L. and the drug glucantime was carried out for one month. The size of the wounds and their healing status were checked at the beginning and end of the study. Also, the histological study was investigated in the groups treated with the mentioned drugs in the absence or presence of different concentrations of the stem and root of the plant and glucantime. The results showed that on the 20th day, the process of reducing the size of the wounds in the positive control group and the groups treated with the essential oil of the stem and root began. The size of the wound in the negative control group and the group treated with 2.5 concentration was significantly higher than other treatment groups (P < 0.05). On the 30th day, the average wound size in the positive control and groups treated with 10 and 20 µg doses was not significant (P > 0.05). The greatest decrease in the size of the wound from the 10th to the 30th day was recorded in the group treated with a concentration of 20 µg of the essential oil of the stem and root so that the size of the wound decreased from 17.29 mm on the 10th day to 5.47 mm on the 30th day. The selected drug glucantime was most effective in reducing the size of the wound, however, the essential oil of the stem and root also was effective in healing and reducing the size of the wound at a concentration of 20 µg.

2/27/2024 12:00:00 AM

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